東京大学医科学研究所 分子遺伝医学分野Division of Molecular and Medical GeneticsCenter for Gene and Cell TherapyThe Institute of Medical Science, The University of Tokyo

論文Publications

  • 2020
  • 2019
  • 2018
  • 2017
  • 2016
  • 2015
  • 2014

Hayashita-Kinoh H, Posadas-Herrera G, Nitahara-Kasahara Y, Kuraoka M, Okada H, Chiyo T, Takeda S, Okada T. Improved transduction of canine X-linked muscular dystrophy with rAAV9-microdystrophin via multipotent mesenchymal stromal cell pretreatment. Mol Ther Methods Clin Dev, in press

Yamaguchi T, Uchida E, Okada T, Ozawa K, Onodera M, Kume A, Shimada T, Takahashi S, Tani K, Nasu Y, Mashimoto T, Mizuguchi H, Mitani K, Maki K. Aspects of gene therapy products using current genome-editing technology in Japan. Hum Gene Ther doi: 10.1089/hum.2020.156, 2020

Terada K, Miyake K, Yamaguchi H, Miyake N, Yamanaka K, Kojima S, Ito E, Inokuchi K, Okada T. TERT and TERC mutations detected in cryptic dyskeratosis congenita suppress telomerase activity. Int J Lab Hematol 42: 316-321, 2020

Hirose T, Hashimoto A, Takahashi Y, Nomura Y, Nitahara-Kasahara Y, Okada T, Takahashi N, Mizumoto S, Yamada S, Nakayama J, Takehana K, Yoshizawa T, Kosho T, Watanabe T. Systemic investigation of the skin in Chst14−/− mice: a model for skin fragility in musculocontractural Ehlers–Danlos syndrome caused by CHST14 mutations (mcEDS-CHST14). Glycobiology 10.1093/glycob/cwaa058, 2020

Ishii A, Okada H, Hayashita-Kinoh H, Shin JH, Tamaoka A, Okada T & Takeda S. rAAV8 and rAAV9-mediated long- term muscle transduction with tacrolimus (FK506) in non-human primates. Mol Ther Methods Clin Dev 18:44-49, 2020

Kuroda S, Miyagawa Y, Sato Y, Yamamoto M, Adachi K, Kinoh H, Goins WF, Cohen JB, Glorioso JC, Taniai N, Yoshida H & OkadaT. Protocol optimization for the production of the non-cytotoxic JΔNI5 herpes simplex virus vector deficient in expression of immediately early genes. Mol Ther Methods Clin Dev 17:612-621, 2020

Shimada S, Yoshizawa T, Takahashi Y, Nitahara-Kasahara Y, Okada T, Nomura Y, Yamanaka H, Kosyo T & Matsumoto K. Backcrossing to an Appropriate Genetic Background Improves the Birth Rate of Carbohydrate Sulfotransferase 14 Gene-deleted Mice. Exp Anim 10.1538/expanim.19-0150, 2020

Shiozawa Lee A, Igarashi T, Kobayashi M, Nakamoto K, Kameya S, Fujishita S, Takahashi H & Okada T. Tyrosine triple mutated AAV2-BDNF gene therapy in an inner retinal injury model induced by intravitreal injection of N–methyl-D-aspartate (NMDA). Mol Vision 26:409-422, 2020

Ito T, Sakai A, Maruyama M, Miyagawa Y, Okada T, Fukayama H & Suzuki H. Dorsal Root Ganglia Homeobox downregulation in primary sensory neurons contributes to neuropathic pain in rats. Mol Pain 16:1744806920904462, 2020

Nakamura-Takahashi A, Tanase T, Matsunaga S, Shintani S, Abe S, Nitahara-Kasahara Y, Watanabe A, Hirai Y, Okada T, Yamaguchi A & Kasahara M. High-Level Expression of Alkaline Phosphatase by Adeno- Associated Virus Vector Ameliorates Pathological Bone Structure in a Hypophosphatasia Mouse Model. Calcif Tissue Int 106: 665-677, 2020

Takahashi K, Igarashi T., Miyake K, Kobayashi M, Katakai Y, Hayashita-Kinoh H, Fujimoto C, Kameya S, Takahashi H, Okada T. The amount of GFP in the anterior chamber after intravitreal injection of triple-mutated self-complementary AAV2 vectors is not affected by previous vitrectomy surgery. J Nippon Med Sch 2020

Igarashi T, Nakamoto K, Kobayashi M, Suzuki H, Igarashi T, Okada T, Takahashi H. Serum brain-derived neurotrophic factor in glaucoma patients in Japan: An observational study. J Nippon Med Sch 10.1272/jnms.JNMS.2020_87-605,

Tomono T, Hirai Y, Chono H, Mineno J, Ishii A, Onodera M, Tamaoka A & Okada T. Infectivity Assessment of Recombinant Adeno-Associated Virus and Wild-Type Adeno-Associated Virus Exposed to Various Diluents and Environmental Conditions. Hum Gene Ther Methods 30:137-143, 2019

Shimazaki K, Kobari T, Oguro K, Yokota H, Kasahara Y, Murashima Y, Watanabe E, Kawai K & Okada T. Hippocampal GAD67 Transduction Using rAAV8 Regulates Epileptogenesis in EL Mice. Mol Ther Methods Clin Dev 13:180-186, 2019

Li H, Okada H, Suzuki S, Sakai K, Izumi H, Matsushima Y, Ichinohe N, Goto YI, Okada T & Inoue K. Gene suppressing therapy for Pelizaeus-Merzbacher disease using artificial microRNA. JCI Insight 4:16, 2019

Zhang B, Nakata M, Lu M, Nakae J, Okada T, Ogawa W & Yada T. Protective role of AgRP neuron's PDK1 against salt-induced hypertension. Biochem Biophys Res Commun 500:910-916, 2018

Yoshizawa T, Mizumoto S, Takahashi Y, Shimada S, Sugahara K, Nakayama J, Takeda S, Nomura Y, Nitahara-Kasahara Y, Okada T, Matsumoto K, Yamada S & Kosho T. Vascular abnormalities in the placenta of Chst14-/- fetuses: implications in the pathophysiology of perinatal lethality of the murine model and vascular lesions in human CHST14/D4ST1 deficiency. Glycobiology 28:80-89, 2018

Tomono T, Hirai Y, Okada H, Miyagawa Y, Adachi K, Sakamoto S, Kawano Y, Chono H, Mineno J, Ishii A, Shimada T, Onodera M, Tamaoka A & Okada T. Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9. Mol Ther Methods Clin Dev 11:180-190, 2018

Sowa K, Nito C, Nakajima M, Suda S, Nishiyama Y, Sakamoto Y, Nitahara-Kasahara Y, Nakamura-Takahashi A, Ueda M, Kimura K & Okada T. Impact of Dental Pulp Stem Cells Overexpressing Hepatocyte Growth Factor after Cerebral Ischemia/Reperfusion in Rats. Mol Ther Methods Clin Dev 10:281-290, 2018

Nito C, Sowa K, Nakajima M, Sakamoto Y, Suda S, Nishiyama Y, Nakamura-Takahashi A, Nitahara-Kasahara Y, Ueda M, Okada T & Kimura K. Transplantation of human dental pulp stem cells ameliorates brain damage following acute cerebral ischemia. Biomed Pharmacother 108:1005-1014, 2018

Nakajima M, Suda S, Sowa K, Sakamoto Y, Nito C, Nishiyama Y, Aoki J, Ueda M, Yokobori S, Yamada M, Yokota H, Okada T & Kimura K. AMPA Receptor Antagonist Perampanel Ameliorates Post-Stroke Functional and Cognitive Impairments. Neuroscience 386:256-264, 2018

Iwasaki Y, Sendo M, Dezaki K, Hira T, Sato T, Nakata M, Goswami C, Aoki R, Arai T, Kumari P, Hayakawa M, Masuda C, Okada T, Hara H, Drucker D J, Yamada Y, Tokuda M & Yada T. GLP-1 release and vagal afferent activation mediate the beneficial metabolic and chronotherapeutic effects of D-allulose. Nat Commun 9:113, 2018

Ikeue R, Nakamura-Takahashi A, Nitahara-Kasahara Y, Watanabe A, Muramatsu T, Sato T & Okada T. Bone-Targeted Alkaline Phosphatase Treatment of Mandibular Bone and Teeth in Lethal Hypophosphatasia via an scAAV8 Vector. Mol Ther Methods Clin Dev 10:361-370, 2018

Fukuoka M, Takahashi M, Fujita H, Chiyo T, Popiel H A, Watanabe S, Furuya H, Murata M, Wada K, Okada T, Nagai Y & Hohjoh H. Supplemental Treatment for Huntington's Disease with miR-132 that Is Deficient in Huntington's Disease Brain. Mol Ther Nucleic Acids 11:79-90, 2018

Takahashi K, Igarashi T, Miyake K, Kobayashi M, Yaguchi C, Iijima O, Yamazaki Y, Katakai Y, Miyake N, Kameya S, Shimada T, Takahashi H & Okada T. Improved Intravitreal AAV-Mediated Inner Retinal Gene Transduction after Surgical Internal Limiting Membrane Peeling in Cynomolgus Monkeys. Mol Ther 25:296-302, 2017

Sakai A, Saitow F, Maruyama M, Miyake N, Miyake K, Shimada T, Okada T & Suzuki H. MicroRNA cluster miR-17-92 regulates multiple functionally related voltage-gated potassium channels in chronic neuropathic pain. Nat Commun 8:16079, 2017

Okawa R, Iijima O, Kishino M, Okawa H, Toyosawa S, Sugano-Tajima H, Shimada T, Okada T, Ozono K, Ooshima T & Nakano K. Gene therapy improves dental manifestations in hypophosphatasia model mice. J Periodontal Res 52:471-478, 2017

Nakata M, Yamamoto S, Okada T & Yada T. AAV-mediated IL-10 gene transfer counteracts inflammation in the hypothalamic arcuate nucleus and obesity induced by high-fat diet. Neuropeptides 62:87-92, 2017

Nakajima M, Nito C, Sowa K, Suda S, Nishiyama Y, Nakamura-Takahashi A, Nitahara-Kasahara Y, Imagawa K, Hirato T, Ueda M, Kimura K & Okada T. Mesenchymal Stem Cells Overexpressing Interleukin-10 Promote Neuroprotection in Experimental Acute Ischemic Stroke. Mol Ther Methods Clin Dev6:102-111, 2017

Miyoshi S, Tezuka T, Arimura S, Tomono T, Okada T & Yamanashi Y. DOK7 gene therapy enhances motor activity and life span in ALS model mice. EMBO Mol Med 9:880-889, 2017

Ito N, Sakai A, Miyake N, Maruyama M, Iwasaki H, Miyake K, Okada T, Sakamoto A & Suzuki H. miR-15b mediates oxaliplatin-induced chronic neuropathic pain through BACE1 down-regulation. Br J Pharmacol 174:386-395, 2017

Tomono T, Hirai Y, Okada H, Adachi K, Ishii A, Shimada T, Onodera M, Tamaoka A & Okada T. Ultracentrifugation-free chromatography-mediated large-scale purification of recombinant adeno-associated virus serotype 1 (rAAV1). Mol Ther Methods Clin Dev3:15058, 2016

Nakata M, Zhang B, Yang Y, Okada T, Shintani N, Hashimoto H & Yada T. High-Fat Diet Augments VPAC1 Receptor-Mediated PACAP Action on the Liver, Inducing LAR Expression and Insulin Resistance. J Diabetes Res 2016:9321395, 2016

Nakata M, Yamamoto S, Okada T, Gantulga D, Okano H, Ozawa K & Yada T. IL-10 gene transfer upregulates arcuate POMC and ameliorates hyperphagia, obesity and diabetes by substituting for leptin. Int J Obes (Lond) 40:425-433, 2016

Nakata M, Gantulga D, Santoso P, Zhang B, Masuda C, Mori M, Okada T & Yada T. Paraventricular NUCB2/Nesfatin-1 Supports Oxytocin and Vasopressin Neurons to Control Feeding Behavior and Fluid Balance in Male Mice. Endocrinology 157:2322-2332, 2016

Nakamura-Takahashi A, Miyake K, Watanabe A, Hirai Y, Iijima O, Miyake N, Adachi K, Nitahara-Kasahara Y, Kinoshita H, Noguchi T, Abe S, Narisawa S, Millan J L, Shimada T & Okada T. Treatment of hypophosphatasia by muscle-directed expression of bone-targeted alkaline phosphatase via self-complementary AAV8 vector. Mol Ther Methods Clin Dev3:15059, 2016

Kuraoka M, Kimura E, Nagata T, Okada T, Aoki Y, Tachimori H, Yonemoto N, Imamura M & Takeda S. Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. Am J Pathol 186:1302-1312, 2016

Igarashi T, Miyake K, Kobayashi M, Kameya S, Fujimoto C, Nakamoto K, Takahashi H, Igarashi T, Miyake N, Iijima O, Hirai Y, Shimada T, Okada T & Takahashi H. Tyrosine triple mutated AAV2-BDNF gene therapy in a rat model of transient IOP elevation. Mol Vis 22:816-826, 2016

Tanokashira D, Motoki K, Minegishi S, Hosaka A, Mamada N, Tamaoka A, Okada T, Lakshmana M K & Araki W. LRP1 Downregulates the Alzheimer's beta-Secretase BACE1 by Modulating Its Intraneuronal Trafficking. eNeuro 2:0006-0015, 2015

Ohtsuka Y, Kanagawa M, Yu C C, Ito C, Chiyo T, Kobayashi K, Okada T, Takeda S & Toda T. Fukutin is prerequisite to ameliorate muscular dystrophic phenotype by myofiber-selective LARGE expression. Sci Rep 5:8316, 2015

Iijima O, Miyake K, Watanabe A, Miyake N, Igarashi T, Kanokoda C, Nakamura-Takahashi A, Kinoshita H, Noguchi T, Abe S, Narisawa S, Millan J L, Okada T & Shimada T. Prevention of Lethal Murine Hypophosphatasia by Neonatal Ex Vivo Gene Therapy Using Lentivirally Transduced Bone Marrow Cells. Hum Gene Ther 26:801-812, 2015

Hironaka K, Yamazaki Y, Hirai Y, Yamamoto M, Miyake N, Miyake K, Okada T, Morita A & Shimada T. Enzyme replacement in the CSF to treat metachromatic leukodystrophy in mouse model using single intracerebroventricular injection of self-complementary AAV1 vector. Sci Rep 5:13104, 2015

Hayashita-Kinoh H, Yugeta N, Okada H, Nitahara-Kasahara Y, Chiyo T, Okada T & Takeda S. Intra-amniotic rAAV-mediated microdystrophin gene transfer improves canine X-linked muscular dystrophy and may induce immune tolerance. Mol Ther 23:627-637, 2015

Dohi T, Miyake K, Aoki M, Ogawa R, Akaishi S, Shimada T, Okada T & Hyakusoku H. Tissue Inhibitor of Metalloproteinase-2 Suppresses Collagen Synthesis in Cultured Keloid Fibroblasts. Plast Reconstr Surg Glob Open 3:e520, 2015

Darambazar G, Nakata M, Okada T, Wang L, Li E, Shinozaki A, Motoshima M, Mori M & Yada T. Paraventricular NUCB2/nesfatin-1 is directly targeted by leptin and mediates its anorexigenic effect. Biochem Biophys Res Commun 456:913-918, 2015

Nitahara-Kasahara Y, Hayashita-Kinoh H, Chiyo T, Nishiyama A, Okada H, Takeda S & Okada T. Dystrophic mdx mice develop severe cardiac and respiratory dysfunction following genetic ablation of the anti-inflammatory cytokine IL-10. Hum Mol Genet 23:3990-4000, 2014

Masamizu Y, Tanaka Y R, Tanaka Y H, Hira R, Ohkubo F, Kitamura K, Isomura Y, Okada T & Matsuzaki M. Two distinct layer-specific dynamics of cortical ensembles during learning of a motor task. Nat Neurosci 17:987-994, 2014

Hira R, Ohkubo F, Masamizu Y, Ohkura M, Nakai J, Okada T & Matsuzaki M. Reward-timing-dependent bidirectional modulation of cortical microcircuits during optical single-neuron operant conditioning. Nat Commun 5:5551, 2014

Arimura S, Okada T, Tezuka T, Chiyo T, Kasahara Y, Yoshimura T, Motomura M, Yoshida N, Beeson D, Takeda S & Yamanashi Y. DOK7 gene therapy benefits mouse models of diseases characterized by defects in the neuromuscular junction. Science 345:1505-1508, 2014

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